Bactron User Group > FAQ Section  »   What should I know about entering the chamber?

1. The rubber cuffs of the sleeve should be secured around your arm as opposed to
clothing. Remove watches, bracelets, etc., as they may damage the cuffs.
2. Insert your hands and forearms into the sleeves. The cuffs must be firmly secured
around your bare forearms. Keep your hands four to six inches away from the
armport doors.
3. Depress the VAC foot pedal (this procedure evacuated both sleeves), and continue
depressing until the sleeves have collapsed completely and firmly around your
forearms.
4. Flush the sleeves with the gas mixture by depressing the GAS foot pedal. Do not
overfill the sleeves. Stop when you feel a small separation of space between your
hands and the sleeves.
5. Repeat steps 3 – 4 three more times to ensure that the sleeves are completely
anaerobic/desired atmosphere before entering the chamber.

NOTE: ALWAYS enter the chamber with both arms simultaneously.

6. Loosen the armport door knobs a few turns and rotate the locking bars to a
horizontal position. The armport doors can now be pushed forward into the chamber
interior and can be secured on the armport door holders.

NOTE: Arm movements that are too forceful may cause the sleeves to dislodge from
the armports.