Bactron User Group > FAQ Section  »   What should I know about exiting the chamber?

1. Before leaving the chamber the incubator doors and the inner Air Lock door must be
closed.
2. Depress the GAS foot pedal to allow the sleeves to fill with gas. This procedure
keeps the sleeves from collapsing as you move outward, making it easier to
reposition the doors.
3. Hold the locking bars in a horizontal position, and close the doors. Rotate the locking
bars to a vertical position, and gently tighten both armport door knobs, just until they
“grab”.
4. Before removing arms from the sleeves:
A) Check to be sure that armport doors are securely sealed by slowly pushing
both arms forward (this action generates a pressure within the sleeves).
B) If the Manometer bubbles when the arms are pushed forward, this indicates
that the doors are improperly sealed. Loosen the armport door knobs again,
remove the doors. Check the O-rings for any particulate matter on the sealing
areas, and repeat Step “A”. If Manometer remains stable, the doors are
sealed properly.
5. When the armport doors are properly sealed, slowly and carefully withdraw both
arms from the sleeves.