Comments on: Bactron Anaerobic Chamber FAQ’s

By: Jed

Jed — Wed, 18 Jan 2012 00:12:34 +0000

The incubator on the Bactron IV-900 can only be accessed by entering into the chamber workspace via the sleeves after making them anaerobic. Yes, you can incubate Erlenmeyer flasks in the incubator, the height of the shelf is the only limitation on the size of flask that will fit.

By: Gail Joseph

Gail Joseph — Fri, 08 Jul 2011 06:21:31 +0000

Hello,
My question is regarding Bactron IV 900. I want to know the process of opening and closing the incubator? do we always have to access it through the working chamber? Also, Along with petri plates, can we incubate Erlenmeyer Flasks in the Incubator?

By: Jed

Jed — Mon, 12 Apr 2010 16:00:50 +0000

Please note that AMG (Anaerobic Mixed Gas) is the mixture that is put together which is made up of 90% nitrogen, 5% hydrogen and 5% CO2. This mixture is the only way to get an anaerobic gas which is the conditions that the system requires.

By: K.C.I

K.C.I — Mon, 12 Apr 2010 09:51:26 +0000

WE’VE RECENTLY BOUGHT A BACTRON I ANAEROBIC CHAMBER. WE ARE HAVING DIFFICULTY OBTAINING THE GASES TO HAVE THE MACHINE RUNNING. IS THERE AN ALTERNATIVE GASES THAT WE CAN USE FOR THIS INSTRUMENT. OUR LOCAL VENDOR CANNOT SUPPLY US WITH THE AMG MIXTURE GASES. THANK YOU.

By: VIMISHA

VIMISHA — Thu, 24 Dec 2009 15:11:01 +0000

Hi, we do have ana. chamber. we do bake our catalyst (pallidium, charcole and dessicant) twice a week. Could you please let me know “how long we have to baked everyday and which temp.?”

Thanks.

Vimisha

By: Catherine

Catherine — Tue, 14 Jul 2009 19:02:48 +0000

Sam,

Congrats on the new chamber. The catalyst is an inexhaustible elemental catalyst. It has no expiration date as long as it is cared for properly. If you follow the SOPs and refresh it every day, you will have years and years of trouble free use.

I am not sure what you mean by 2-h? DO NOT purge your system with Hydrogen! You can use an anaerobic mixed gas. You will use 300-400 psi of gas from the contents gauge of your tank, depending on your model.

Catherine

By: Juan Maldonado

Juan Maldonado — Tue, 14 Jul 2009 17:12:20 +0000

Sam,

The catalyst will never wear out as long as it is stored in a cool dry place and cleaned regularly. Prior to putting the catalyst back in use make sure that it is baked at 160 degrees Celsius. The usual procedure for the catalyst is to have one in the chamber while you bake out the other and they should be switched daily. After you have initially purged the unit the chamber should remain anaerobic as long as the following conditions are kept:

- The catalyst is changed out daily
- No oxygen is accidentally introduced to the chamber
- The chamber has adequate positive pressure
- The chamber atmosphere contains 5% Hydrogen, which is used to scrub the residual oxygen out by the catalyst.

Juan Maldonado
800-322-4897 x.12

By: Sam

Sam — Tue, 14 Jul 2009 12:50:46 +0000

Hi,

I need to know the shelflife of the catalyst and how to store it when not in use please.

And please let me know after the first time purging the system to make it anaerobic (2-h gassing, is that cirrect?), do I need to regularly purge the chamber (not in case of leaking, as a routine)? and if yes, how often?

I appreciate your prompt reply in advance.

Sincerely,

Sam